ATG7 Rabbit Polyclonal Antibody

CAT#: TA324635

Rabbit polyclonal ATG7 Antibody

Product Images

Western blot analysis of anti-hAPG7L-R509 Pab (Cat. #TA324635) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-R509 (arrow) was detected using the purified Pab.

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  The anti-APG7L Pab (Cat. #TA324635) is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L (arrow) was detected using the purified Pab.

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  APG7L Antibody (R509) (Cat. #TA324635) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine, then incubated with TA324635 ATG7 primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:200, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse (Predicted: Rat)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77960 Da
• Gene Name: autophagy related 7
• Database Link:
  NP_006386
  Entrez Gene 74244 MouseEntrez Gene 312647 RatEntrez Gene 10533 Human
  UniProt ID O95352
• Synonyms: APG7-LIKE; APG7L; GSA7

Reference Data

• Protein Pathways: Regulation of autophagy