ATG9A Rabbit Polyclonal Antibody

CAT#: TA324637

Rabbit polyclonal ATG9A Antibody (C-term)

Product Images

Western blot analysis of anti-Autophagy APG9L1 Antibody (C-term) (Cat.#TA324637) in A2058 and A375 cell line lysates (35ug/lane). APG9L1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine, then incubated with TA324637 ATG9A (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717-746 amino acids from the C-terminal region of human ATG9A.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 94447 Da
• Gene Name: autophagy related 9A
• Database Link:
  NP_001070666
  Entrez Gene 79065 Human
  UniProt ID Q7Z3C6
• Synonyms: APG9L1; mATG9; MGD3208

Reference Data

• Protein Families: Transmembrane