ATG16L1 Rabbit Polyclonal Antibody

CAT#: TA324639

Rabbit polyclonal ATG16L Antibody

Product Images

Western blot of APG16L (L92) Pab (Cat. #TA324639) in mouse brain tissue lysate.

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  APG16L Antibody (Cat. #TA324639) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).

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  Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

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  Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Immunofluorescent analysis of U251 cells, using ATG16L Antibody (Cat. #TA324639). U251 cells (right) were treated with Chloroquine (50 μM, 16h). TA324639 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:25, WB: 1:1000, IHC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68265 Da
• Gene Name: autophagy related 16 like 1
• Database Link:
  NP_942593
  Entrez Gene 77040 MouseEntrez Gene 55054 Human
  UniProt ID Q676U5
• Synonyms: APG16L; ATG16A; ATG16L; IBD10; WDR30