NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: TA324641

Rabbit polyclonal NFKBp65 Antibody (C-term S536)

Product Images

NFKBp65 Antibody (pS536) (Cat. #TA324641) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).

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  IF image of U251 cell stained with NFKBp65 Antibody (C-termS536) (Cat#TA324641).U251 cells were incubated with NFKBp65 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This NFKBp65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-539 amino acids from the C-terminal region of human NFKBp65.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60219 Da
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  NP_068810
  Entrez Gene 19697 MouseEntrez Gene 5970 Human
  UniProt ID Q04206
• Synonyms: NFKB3; p65

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway