LAMP1 Rabbit Polyclonal Antibody

CAT#: TA324642

Rabbit polyclonal LAMP1 Antibody (N-term)

Product Images

Western blot analysis of lysates from Hela, HT1080 cell line and human placenta tissue (from left to right), using LAMP1 Antibody (N-term) (Cat. #TA324642).TA324642 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *LAMP1 antibody (N-term) (Cat.#TA324642), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cells stained with LAMP1 (N-term) antibody. U251 cells were treated with Chloroquine, then incubated with TA324642 LAMP1 (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). LAMP1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:200, IHC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LAMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the N-terminal region of human LAMP1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44882 Da
• Gene Name: lysosomal associated membrane protein 1
• Database Link:
  NP_005552
  Entrez Gene 16783 MouseEntrez Gene 3916 Human
  UniProt ID P11279
• Synonyms: CD107a; LAMPA; LGP120

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Lysosome