KLF4 Rabbit Polyclonal Antibody

CAT#: TA324725

Rabbit polyclonal KLF4 Antibody

Product Images

Western blot analysis of KLF4-S457 (Cat.# TA324725) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.

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  IF image of HeLa cells stained with TA324725 KLF4 (S457) antibody. HeLa cells were incubated with TA324725 KLF4 (S457) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.

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  IF image of SY5Y cells stained with TA324725 KLF4 (S457) antibody. SY5Y cells were incubated with TA324725 KLF4 (S457) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.

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  Immunofluorescent analysis of U251 cells, using KLF4 Antibody (Cat. #TA324725). TA324725 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-504 amino acids from human KLF4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54671 Da
• Gene Name: Kruppel-like factor 4 (gut)
• Database Link:
  NP_004226
  Entrez Gene 16600 MouseEntrez Gene 114505 RatEntrez Gene 9314 Human
  UniProt ID O43474
• Synonyms: EZF; GKLF

Reference Data

• Protein Families: Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors