Vimentin (VIM) Rabbit Polyclonal Antibody

CAT#: TA324729

Rabbit polyclonal Vimentin Antibody (C-term)

Product Images

Western blot analysis of Vimentin antibody (C-term) (Cat.#TA324729) in NCI-H460 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.

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  Vimentin Antibody (C-term) (Cat. #TA324729)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of SY5Y cells stained with Vimentin (C-term) antibody. SY5Y cells were incubated with TA324729 Vimentin (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).

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  Vimentin Antibody (C-term) (Cat. #TA324729) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53652 Da
• Gene Name: vimentin
• Database Link:
  NP_003371
  Entrez Gene 22352 MouseEntrez Gene 81818 RatEntrez Gene 705289 MonkeyEntrez Gene 7431 Human
  UniProt ID P08670
• Synonyms: CTRCT30; HEL113

Reference Data

• Protein Families: ES Cell Differentiation/IPS