Nucleophosmin (NPM1) Rabbit Polyclonal Antibody

CAT#: TA324742

Rabbit polyclonal NPM1 Antibody (C-term)

Product Images

NPM1 Antibody (C-term) (Cat. #TA324742) western blot analysis in Hela, Jurkat cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).

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  NPM1 Antibody (C-term) (TA324742)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of A549 cell stained with NPM1 Antibody (C-term) (Cat#TA324742/SH090717BE).A549 cells were incubated with NPM1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.

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  NPM1 Antibody (C-term) (Cat. #TA324742) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, FC: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32575 Da
• Gene Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Database Link:
  NP_001032827
  Entrez Gene 4869 Human
  UniProt ID P06748
• Synonyms: B23; NPM

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors