Hsp60 (HSPD1) Rabbit Polyclonal Antibody

CAT#: TA324748

Rabbit polyclonal anti-HSPD1(HSP60) antibody(Center), Loading control

Product Images

HSPD1 Antibody (Center) (Cat. #TA324748) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).

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  IF image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria.

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  IF image of Hela cell stained with HSPD1 Antibody (Center) (Cat#TA324748). Hela cells were incubated with HSPD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). HSPD1 immunoreactivity is localized to Mitochondria significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF: 1:10 - 50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61055 Da
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  NP_002147
  Entrez Gene 15510 MouseEntrez Gene 63868 RatEntrez Gene 3329 Human
  UniProt ID P10809
• Synonyms: CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Type I diabetes mellitus