APRT Rabbit Polyclonal Antibody

CAT#: TA324753

Rabbit polyclonal APRT Antibody (N-term)

Product Images

APRT Antibody (N-term) (Cat. #TA324753) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of A549 cell stained with APRT Antibody (N-term) (Cat#TA324753/SA091009AF).A549 cells were incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.

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  Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human APRT.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19608 Da
• Gene Name: adenine phosphoribosyltransferase
• Database Link:
  NP_000476
  Entrez Gene 353 Human
  UniProt ID P07741
• Synonyms: AMP; APRTD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism