Peroxiredoxin 2 (PRDX2) Rabbit Polyclonal Antibody

CAT#: TA324756

Rabbit polyclonal PRDX2 Antibody (C-term)

Product Images

Western blot analysis of PRDX2 Antibody (C-term) (Cat. #TA324756) in MDA-MB231, 293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab. (2ug/ml)

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  Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody (C-term) (Cat. #TA324756). TA324756 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of Hela cell stained with PRDX2 Antibody (C-term) (Cat#TA324756).Hela cells were incubated with PRDX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).PRDX2 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21892 Da
• Gene Name: peroxiredoxin 2
• Database Link:
  NP_005800
  Entrez Gene 21672 MouseEntrez Gene 29338 RatEntrez Gene 716665 MonkeyEntrez Gene 7001 Human
  UniProt ID P32119
• Synonyms: HEL-S-2a; NKEF-B; NKEFB; PRP; PRX2; PRXII; PTX1; TDPX1; TPX1; TSA

Reference Data

• Protein Families: Druggable Genome