p53 (TP53) Rabbit Polyclonal Antibody

CAT#: TA324776

Rabbit polyclonal Phospho-p53(T18) Antibody

Product Images

Western blot analysis of anti-Phospho-p53-T18 Pab (cat# TA324776) in, from left to right, A2058, Ramos, mouse lung, mouse testis, and HL60 cell line lysates. Phospho-p53-T18 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Dot blot analysis of anti-hp53-T18 Phospho-specific Pab (Cat. #TA324776) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.

Specifications

Product Data

• Applications: Dot, IHC, WB
• Recommended Dilution: DB: 1:500, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This p53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43653 Da
• Gene Name: tumor protein p53
• Database Link:
  NP_000537
  Entrez Gene 22059 MouseEntrez Gene 716170 MonkeyEntrez Gene 7157 Human
  UniProt ID P04637
• Synonyms: BCC7; LFS1; P53; TRP53

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway