ERK1 (MAPK3) Rabbit Polyclonal Antibody

CAT#: TA324783

Rabbit polyclonal Bi-Phospho-ERK1/2(T202/Y204) Antibody

Product Images

Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK (T202/Y204) (Cat.#TA324783). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1) (2 ug/lane).

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  Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK (T202/Y204) (Cat.#TA324783). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1) (2 ug/lane).

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  Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody (Cat.#TA324783) on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: DB: 1:500, WB: 1:1000
• Reactivities: Human (Predicted: Mouse, Rat, Drosophila)
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ERK1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43136 Da
• Gene Name: mitogen-activated protein kinase 3
• Database Link:
  NP_002737
  Entrez Gene 26417 MouseEntrez Gene 50689 RatEntrez Gene 5595 Human
  UniProt ID P27361
• Synonyms: ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway