p53 (TP53) Rabbit Polyclonal Antibody

CAT#: TA324825

Rabbit polyclonal p53 Antibody (S315)

Product Images

p53 Antibody (S315) (Cat. #TA324825) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the p53 antibody detected the p53 protein (arrow).

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with p53 Antibody (S315) (Cat.#TA324825), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cell stained with p53 Antibody (S315) (Cat#TA324825).U251 cells were incubated with p53 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). p53 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from human p53.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43653 Da
• Gene Name: tumor protein p53
• Database Link:
  NP_000537
  Entrez Gene 716170 MonkeyEntrez Gene 7157 Human
  UniProt ID P04637
• Synonyms: BCC7; LFS1; P53; TRP53

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway