AGR2 Rabbit Polyclonal Antibody

CAT#: TA324827

Rabbit polyclonal Anterior Gradient 2 (AGR2) Antibody (N-term)

Product Images

AGR2 Antibody (N-term) (Cat. #TA324827) western blot analysis in HT29, MCF-7, ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat.#TA324827), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of A549 cell stained with AGR2 Antibody (N-term) (Cat#TA324827).A549 cells were incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19979 Da
• Gene Name: anterior gradient 2, protein disulphide isomerase family member
• Database Link:
  NP_006399
  Entrez Gene 10551 Human
  UniProt ID O95994
• Synonyms: AG2; GOB-4; HAG-2; HEL-S-116; PDIA17; XAG-2

Reference Data

• Protein Families: Secreted Protein