YB1 (YBX1) Rabbit Polyclonal Antibody

CAT#: TA324830

Rabbit polyclonal YBX1 Antibody (C-term)

Product Images

Western blot analysis of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (C-term) (Cat. #TA324830). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).

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  YBX1 Antibody (C-term) (Cat. #TA324830) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow).

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  IF image of U251 cell stained with YBX1 Antibody (C-term) (Cat#TA324830).U251 cells were incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). YBX1 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Bovine, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35924 Da
• Gene Name: Y-box binding protein 1
• Database Link:
  NP_004550
  Entrez Gene 22608 MouseEntrez Gene 500538 RatEntrez Gene 4904 Human
  UniProt ID P67809
• Synonyms: BP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors