Tuberin (TSC2) Rabbit Polyclonal Antibody

CAT#: TA324836

Rabbit polyclonal Tuberin (TSC2) Antibody (S1798)

Product Images

Western blot analysis of TSC2-pS1798 (Cat. #TA324836) in Ramos cell line lysates (35ug/lane). TSC2 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) (Cat.#TA324836), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of Hela cell stained with Tuberin (TSC2) Antibody (S1798) (Cat#TA324836).Hela cells were incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Tuberin (TSC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1776-1805 amino acids from human Tuberin (TSC2).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 200608 Da
• Gene Name: tuberous sclerosis 2
• Database Link:
  NP_000539
  Entrez Gene 7249 Human
  UniProt ID P49815
• Synonyms: LAM; PPP1R160; TSC4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, p53 signaling pathway