Parkin (PARK2) Rabbit Polyclonal Antibody

CAT#: TA324838

Rabbit polyclonal Parkin Antibody (N-term)

Product Images

Western blot analysis of lysate from SH-SY5Y cell line, using Park2 Antibody (N-term) (Cat. #TA324838). TA324838 was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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  Parkin Antibody (N-term) (TA324838)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of Parkin Antibody (N-term) (Cat#TA324838) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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  Parkin Antibody (N-term) (Cat. #TA324838) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human Parkin.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51641 Da
• Gene Name: parkin RBR E3 ubiquitin protein ligase
• Database Link:
  NP_004553
  Entrez Gene 5071 Human
  UniProt ID O60260
• Synonyms: AR-JP; LPRS2; PDJ; PRKN

Reference Data

• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis