Parkin (PARK2) Rabbit Polyclonal Antibody

CAT#: TA324839

Rabbit polyclonal Parkin Antibody (C-term)

Product Images

Park2 Antibody (C-term) (Cat. #TA324839) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).

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  The anti-Parkin (C-term) Pab (Cat. #TA324839) is used in Western blot to detect Parkin in mouse kidney tissue lysate.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) (Cat.#TA324839), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of Parkin Antibody (C-term) (Cat#TA324839) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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  Parkin Antibody (C-term) (Cat. #TA324839) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, FC: 1:10~50, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-417 amino acids from the C-terminal region of human Parkin.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51641 Da
• Gene Name: parkin RBR E3 ubiquitin protein ligase
• Database Link:
  NP_004553
  Entrez Gene 50873 MouseEntrez Gene 5071 Human
  UniProt ID O60260
• Synonyms: AR-JP; LPRS2; PDJ; PRKN

Reference Data

• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis