TATA binding protein (TBP) Rabbit Polyclonal Antibody

CAT#: TA324859

Rabbit polyclonal anti-TBP antibody, Loading control

Product Images

TBP Antibody (Center) (Cat. #TA324859) western blot analysis in K562, U251 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cell stained with TBP Antibody (Center) (Cat#TA324859).U251 cells were incubated with TBP primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).TBP immunoreactivity is localized to Nucleus significantly.

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  TBP Antibody (Center) (Cat. #TA324859) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50 - 100, IF 1:10 - 50, FC 1:10 - 50
• Reactivities: Human (Predicted: Mouse, Zebrafish, Bovine, Chicken, Monkey, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the Central region of human TBP.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37698 Da
• Gene Name: TATA-box binding protein
• Database Link:
  NP_003185
  Entrez Gene 21374 MouseEntrez Gene 696258 MonkeyEntrez Gene 6908 Human
  UniProt ID P20226
• Synonyms: GTF2D; GTF2D1; HDL4; SCA17; TFIID

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors, Huntington's disease