MOB4A (MOB1B) Rabbit Polyclonal Antibody

CAT#: TA324899

Rabbit polyclonal MOB4A Antibody (C-term)

Product Images

MOB4A Antibody (C-term) (Cat.# TA324899) western blot analysis in Daudi, K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).

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  Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term) (Cat. #TA324899). TA324899 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) (Cat.#TA324899), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of NIH/3T3 cell stained with MOB4A Antibody (C-term) (Cat#TA324899).NIH/3T3 cells were incubated with MOB4A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).MOB4A immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25091 Da
• Gene Name: MOB kinase activator 1B
• Database Link:
  NP_775739
  Entrez Gene 68473 MouseEntrez Gene 360920 RatEntrez Gene 92597 Human
  UniProt ID Q7L9L4
• Synonyms: MATS2; MOB4A; MOBKL1A