Carbonic Anhydrase II (CA2) Rabbit Polyclonal Antibody

CAT#: TA324917

Rabbit polyclonal CA2 Antibody (N-term)

Product Images

CA2 Antibody (N-term) (Cat.# TA324917) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the CA2 antibody detected the CA2 protein (arrow).

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  Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term) (Cat. #TA324917). TA324917 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  IF image of Hela cell stained with CA2 Antibody (N-term) (Cat#TA324917).Hela cells were incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). CA2 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29246 Da
• Gene Name: carbonic anhydrase 2
• Database Link:
  NP_000058
  Entrez Gene 760 Human
  UniProt ID P00918
• Synonyms: CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism