EN2 Rabbit Polyclonal Antibody

CAT#: TA324936

Rabbit polyclonal EN2 Antibody (C-term)

Product Images

Western blot analysis of EN2 antibody (C-term) (Cat.# TA324936) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.

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  IF image of Hela cell stained with EN2 Antibody (C-term) (Cat#TA324936). Hela cells were incubated with EN2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34211 Da
• Gene Name: engrailed homeobox 2
• Database Link:
  NP_001418
  Entrez Gene 13799 MouseEntrez Gene 2020 Human
  UniProt ID P19622
• Synonyms: AUTS1; AUTS10; engrailed-2; engrailed homeobox 2; engrailed homolog 2

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS