Her2 (ERBB2) Rabbit Polyclonal Antibody

CAT#: TA324958

Rabbit polyclonal ERBB2 Antibody(N-term)

Product Images

Western blot analysis of ErbB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HER2/ErbB2 antibody (N-term) (Cat.#TA324958), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow cytometric analysis of MCF-7 cells using HER2/ErbB2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human ERBB2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 137910 Da
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  NP_001005862
  Entrez Gene 13866 MouseEntrez Gene 24337 RatEntrez Gene 2064 Human
  UniProt ID P04626
• Synonyms: CD340; HER-2; HER2; MLN 19; NEU; neu; NGL; TKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer