FGFR4 Rabbit Polyclonal Antibody

CAT#: TA324961

Rabbit polyclonal FGFR4 Antibody (N-term)

Product Images

FGFR4 Antibody (E39) (Cat. #TA324961) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FGFR4 antibody detected the FGFR4 protein (arrow).

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  Western blot analysis of lysates from SH-SY5Y 293, Raji cell line (from left to right), using FGFR4 Antibody (N-term) (Cat. #TA324961). TA324961 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of HeLa cells stained with FGFR4 (N-term) antibody. HeLa cells were incubated with TA324961 FGFR4 (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000160867).

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  Flow cytometric analysis of WiDr cells using FGFR4 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:200, FC: 1:10~50, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87954 Da
• Gene Name: fibroblast growth factor receptor 4
• Database Link:
  NP_002002
  Entrez Gene 2264 Human
  UniProt ID P22455
• Synonyms: CD334; JTK2; TKF

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, MAPK signaling pathway, Regulation of actin cytoskeleton