LCK Rabbit Polyclonal Antibody

CAT#: TA324976

Rabbit polyclonal LCK Antibody (N-term)

Product Images

Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate (from left to right), using LSK Antibody (I37) (Cat. #TA324976). TA324976 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Confocal immunofluorescent analysis of LCK Antibody (N-term) (Cat#TA324976) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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  LCK Antibody (N-term) (Cat. #TA324976) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human LCK.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58001 Da
• Gene Name: LCK proto-oncogene, Src family tyrosine kinase
• Database Link:
  NP_001036236
  Entrez Gene 16818 MouseEntrez Gene 3932 Human
  UniProt ID P06239
• Synonyms: IMD22; LSK; p56lck; pp58lck; YT16

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway