SMAD4 Rabbit Polyclonal Antibody

CAT#: TA324984

Rabbit polyclonal SMAD4 Antibody (C-term)

Product Images

Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) (Cat. #TA324984). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

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  Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of Hela cell stained with SMAD4 Antibody (C-term) (Cat#TA324984).Hela cells were incubated with SMAD4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

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  SMAD4 Antibody (C-term) (Cat. #TA324984) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60439 Da
• Gene Name: SMAD family member 4
• Database Link:
  NP_005350
  Entrez Gene 17128 MouseEntrez Gene 50554 RatEntrez Gene 4089 Human
  UniProt ID Q13485
• Synonyms: DPC4; JIP; MADH4; MYHRS

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway