HLAB (HLA-B) Rabbit Polyclonal Antibody

CAT#: TA325053

Rabbit polyclonal HLA-B Antibody (N-term)

Product Images

Western blot analysis of HLA-B Antibody (N-term) (Cat. #TA325053) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.

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  Western blot analysis of HLA-B Antibody (N-term) (Cat. #TA325053) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.

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  Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (Cat. #TA325053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.

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  Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term) (Cat. #TA325053). TA325053 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Western blot analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term) (Cat. #TA325053). TA325053 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

• HLA-B Antibody (N-term) (Cat.#TA325053) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40428 Da
• Gene Name: major histocompatibility complex, class I, B
• Database Link:
  NP_005505
  Entrez Gene 3106 Human
  UniProt ID P01889
• Synonyms: AS; HLAB; SPDA1

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis