EWSR1 Rabbit Polyclonal Antibody

CAT#: TA325075

Rabbit polyclonal EWSR1 Antibody (C-term)

Product Images

Western blot analysis of EWSR1 Antibody (C-term) (Cat. #TA325075) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab.

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  IF image of U251 cell stained with EWSR1 Antibody (C-term) (Cat#TA325075).U251 cells were incubated with EWSR1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).EWSR1 immunoreactivity is localized to Nucleus significantly.

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  EWSR1 Antibody (C-term) (Cat. #TA325075) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68478 Da
• Gene Name: EWS RNA binding protein 1
• Database Link:
  NP_053733
  Entrez Gene 14030 MouseEntrez Gene 2130 Human
  UniProt ID Q01844
• Synonyms: bK984G1.4; EWS; EWS-FLI1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors