UHRF1 Rabbit Polyclonal Antibody

CAT#: TA325077

Rabbit polyclonal UHRF1 Antibody (Center)

Product Images

UHRF1 Antibody (Center) (Cat. #TA325077) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UHRF1 antibody detected the UHRF1 protein (arrow).

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  IF image of Hela cell stained with UHRF1 Antibody (Center) (Cat#TA325077).Hela cells were incubated with UHRF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). UHRF1 immunoreactivity is localized to Nucleus significantly.

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  UHRF1 Antibody (Center) (Cat.#TA325077) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This UHRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-257 amino acids from the Central region of human UHRF1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89814 Da
• Gene Name: ubiquitin like with PHD and ring finger domains 1
• Database Link:
  NP_001041666
  Entrez Gene 29128 Human
  UniProt ID Q96T88
• Synonyms: hNP95; hUHRF1; huNp95; ICBP90; Np95; RNF106

Reference Data

• Protein Families: Druggable Genome, Transcription Factors