TBX1 Rabbit Polyclonal Antibody

CAT#: TA325103

Rabbit polyclonal TBX1 Antibody (C-term)

Product Images

TBX1 Antibody (C-term) (Cat.# TA325103) western blot analysis in HLVEC cell line lysates (35ug/lane).This demonstrates the TBX1 antibody detected the TBX1 protein (arrow).

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  Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody (C-term) (Cat. #TA325103). TA325103 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of A2058 cell stained with TBX1 Antibody (C-term) (Cat#TA325103). A2058 cells were incubated with TBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). TBX1 immunoreactivity is localized to nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human TBX1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43133 Da
• Gene Name: T-box 1
• Database Link:
  NP_005983
  Entrez Gene 6899 Human
  UniProt ID O43435
• Synonyms: CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS

Reference Data

• Protein Families: Transcription Factors