SMAD3 Rabbit Polyclonal Antibody

CAT#: TA325124

Rabbit polyclonal SMAD3-S208 Antibody

Product Images

SMAD3 Antibody (S208) (Cat. #TA325124) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).

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  SMAD3-S208 Antibody (cat. #TA325124) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of SMAD3-S208 Antibody (Cat#TA325124) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

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  SMAD3-S208 Antibody (Cat. #TA325124) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human, Mouse (Predicted: Rat, Chicken, Pig)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48081 Da
• Gene Name: SMAD family member 3
• Database Link:
  NP_005893
  Entrez Gene 17127 MouseEntrez Gene 25631 RatEntrez Gene 4088 Human
  UniProt ID P84022
• Synonyms: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway