Caspase 3 (CASP3) Rabbit Polyclonal Antibody

CAT#: TA325294

Rabbit Polyclonal Caspase 3 (Ser150) Antibody (Phospho-specific)

Product Images

Western blot analysis of Caspase 3 phosphorylation expression in Etoposide treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human Caspase 3 around the phosphorylation site of Serine 150
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: caspase 3
• Database Link:
  NP_004337
  Entrez Gene 12367 MouseEntrez Gene 25402 RatEntrez Gene 836 Human
  UniProt ID P42574
• Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
• Synonyms: CPP32; CPP32B; SCA-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis