CDC25B Rabbit Polyclonal Antibody

CAT#: TA325331

Rabbit Polyclonal CDC25B (Ser323) Antibody (Phospho-specific)

Product Images

Western blot analysis of CDC25B phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human CDC25B around the phosphorylation site of Serine 323
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: cell division cycle 25B
• Database Link:
  NP_004349
  Entrez Gene 12531 MouseEntrez Gene 171103 RatEntrez Gene 994 Human
  UniProt ID P30305
• Background: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
• Synonyms: cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation