E Cadherin (CDH1) Rabbit Polyclonal Antibody

CAT#: TA325337

Rabbit Polyclonal E-cadherin Antibody

Product Images

Western blot analysis of extracts from 293 cells, using E-cadherin Antibody. The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-cadherin Antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human E-cadherin
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cadherin 1
• Database Link:
  NP_004351
  Entrez Gene 999 Human
  UniProt ID P12830
• Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, ?-catenin (also called plakoglobin), and p120 catenin. β-catenin and ?-catenin associate with a-catenin, which links the cadherin-catenin complex to the actin cytoskeleton . While β- and ?-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis .
• Synonyms: Arc-1; CD324; CDHE; ECAD; LCAM; UVO

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer