Chk1 (CHEK1) Rabbit Polyclonal Antibody

CAT#: TA325371

Rabbit Polyclonal Chk1(Ser280) Antibody (Phospho-specific)


USD 345.00

4 Weeks*

Size
    • 100 ul

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Specifications

Product Data
Applications WB
Recommended Dilution WB: 1:500-1:2000
Reactivities Human
Modifications Phospho-specific
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen The antiserum was produced against a synthesized A synthesized peptide derived from human Chk1 around the phosphorylation site of Sersine 280.
Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration lot specific
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name checkpoint kinase 1
Background Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A.
Synonyms CHK1
Reference Data
Protein Families Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways Cell cycle, p53 signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.