EIF4E Rabbit Polyclonal Antibody

CAT#: TA325446

Rabbit Polyclonal eIF4E (Ser209) Antibody (Phospho-specific)

Product Images

Western blot analysis of eIF4E phosphorylation expression in FBS treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human eIF4E around the phosphorylation site of Serine 209
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  NP_001124150
  Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human
  UniProt ID P06730
• Background: eIF4E a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Synonyms: AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway