IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

CAT#: TA325730

Rabbit Polyclonal I?B-a(Ser32/Ser36) Antibody (Phospho-specific)

Product Images

Western blot analysis of extracts from COS7 cells, using Phospho-IkappaB-alpha (Ser32/Ser36) Antibody. The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human I?B-a around the phosphorylation site of Sersine 32/Sersine 36
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NFKB inhibitor alpha
• Database Link:
  NP_065390
  Entrez Gene 18035 MouseEntrez Gene 25493 RatEntrez Gene 4792 Human
  UniProt ID P25963
• Background: The NF-?B/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory I?B proteins . Activation occurs via phosphorylation of I?Ba at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-?B. I?Ba phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate I?B at these activating sites have been identified.
• Synonyms: IKBA; MAD-3; NFKBI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway