SYK Rabbit Polyclonal Antibody

CAT#: TA325921

Rabbit Polyclonal SYK Antibody

Product Images

Western blot analysis of extracts from Jurkat cells, using SYK Antibody. The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded breast carcinoma tissue, using SYK Antibody.?

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human SYK
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: spleen tyrosine kinase
• Database Link:
  NP_003168
  Entrez Gene 20963 MouseEntrez Gene 25155 RatEntrez Gene 6850 Human
  UniProt ID P43405
• Background: The Src-related protein tyrosine kinases Lck p56 and Fyn p59 are critically involved in T cell antigen receptor (TCR)/CD3-triggered activation. T lymphocytes also express a second class of non-receptor protein tyrosine kinases which includes Syk p70 and ZAP p72. These kinases resemble the Src family protein tyrosine kinases in that they have a C-terminal catalytic domain, but differ in that they are characterized by two SH2 domains but no SH3 domains. Evidence for the involvement of the Syk/ZAP family proteins in T cell activation was suggested by the finding that Syk p72 kinase fused to the transmembrane and extracellular domains of CD7 and DC16, respectively, can induce complete T cell activation. In contrast, the ZAP p70 kinase was insufficient unless it was coaggregated with a Fyn p59-containing chimera,suggesting that regulation of ZAP p70 activity may require a functional interaction with Src family kinases.
• Synonyms: p72-Syk

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity