Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA325968

Rabbit Polyclonal Vinculin (Tyr821) Antibody (Phospho-specific)

Product Images

Western blot analysis of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyrosine 821
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 123 kDa
• Gene Name: vinculin
• Database Link:
  NP_003364
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and a-actinin. In addition, vinculin, Talin and a-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression.
• Synonyms: CMD1W; CMH15; HEL114; MV; MVCL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton