Vimentin (VIM) Rabbit Polyclonal Antibody

CAT#: TA325975

Rabbit Polyclonal Vimentin (Ser56) Antibody (Phospho-specific)

Product Images

Western blot analysis of Vimentin phosphorylation expression in Nocodazole treated A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human Vimentin around the phosphorylation site of Serine 56
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: vimentin
• Database Link:
  NP_003371
  Entrez Gene 7431 Human
  UniProt ID P08670
• Background: VIM is an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
• Synonyms: CTRCT30; HEL113

Reference Data

• Protein Families: ES Cell Differentiation/IPS