B Raf (BRAF) Rabbit Polyclonal Antibody

CAT#: TA326162

Rabbit polyclonal B-RAF (Phospho-Ser446) antibody

Product Images

Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from K562 cells (Lane 2), JK cells (Lane 3) and 293 cells (Lane 4), using B-RAF (Phospho-Ser446) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: B-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  NP_004324
  Entrez Gene 109880 MouseEntrez Gene 673 Human
  UniProt ID P15056
• Synonyms: B-raf; B-RAF1; BRAF1; NS7; RAFB1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction