Nucleophosmin (NPM1) Rabbit Polyclonal Antibody

CAT#: TA326172

Rabbit polyclonal NPM (Phospho-Thr199) antibody

Product Images

Western blot analysis of extracts from HepG2 cells, using NPM (Phospho-Thr199) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using NPM (Phospho-Thr199) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NPM (Phospho-Thr199) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000 IF: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:60000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 199 (R-D-TP-P-A).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Database Link:
  NP_954654
  Entrez Gene 18148 MouseEntrez Gene 25498 RatEntrez Gene 4869 Human
  UniProt ID P06748
• Synonyms: B23; NPM

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors