Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: 3B9.E6]

CAT#: TA326376

Mouse monoclonal Hsp40 (Hdj1) Antibody

Product Images

Hsp40 HDJ1 visulaized using the antibody

Specifications

Product Data

• Clone Name: 3B9.E6
• Applications: IF
• Recommended Dilution: WB: 1:2000
• Reactivities: Human, Mouse, Rat. Other species not tested yet
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Protein Hsp40 (Hdj1)
• Formulation: PBS pH7.2, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DnaJ heat shock protein family (Hsp40) member B1
• Database Link:
  NP_006136
  Entrez Gene 81489 MouseEntrez Gene 361384 RatEntrez Gene 3337 Human
  UniProt ID P25685
• Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) . The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain . Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function . HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins . The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 . Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. .
• Synonyms: Hdj1; Hsp40; HSPF1; RSPH16B; Sis1
• Note: Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.