GRP94 (HSP90B1) Rat Monoclonal Antibody [Clone ID: 9G10]

CAT#: TA326419

Mouse monoclonal GRP94 Antibody

Product Images

Western blot analysis of Grp94 using Hela cell lysate at 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: 9G10
• Applications: WB
• Recommended Dilution: 0.5ug/ml was sufficient for detection of Grp94 in 20ug of HeLa lysate.
• Reactivities: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Pig, Horse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Grp94 isolated from chicken oviducts
• Formulation: PBS pH7.2, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein 90kDa beta family member 1
• Database Link:
  NP_003290
  Entrez Gene 22027 MouseEntrez Gene 362862 RatEntrez Gene 699568 MonkeyEntrez Gene 7184 Human
  UniProt ID P14625
• Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion . Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 . Both Hsp90 and Grp94 are calcium binding proteins . Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity . Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. . Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER .
• Synonyms: ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
• Note: Detects a 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74, or GrpE from E.coli.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer