Bcl2 Binding component 3 (BBC3) Rabbit Polyclonal Antibody

CAT#: TA326460

Rabbit Polyclonal Anti-PUMA (NT) Antibody

Product Images

Western blot analysis of PUMA expression in K562 cell lysates using 1:500 dilution of the antibody

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  IHC analysis of PUMA in human breast carcinoma using a 1:100 dilution of the antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500, ICC: 1:100
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal amino acids of human PUMA
• Formulation: PBS with 0.02% sodium azide
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2 binding component 3
• Database Link:
  NP_001120712
  Entrez Gene 27113 Human
  UniProt ID Q96PG8
• Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse . PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
• Synonyms: JFY-1; JFY1; PUMA
• Note: Detects a ~23kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kDa bands may be seen in some instances, possibly corresponding to PUMAβ.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, p53 signaling pathway