HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: ESEE122]

CAT#: TA326476

Mouse Monoclonal Anti-HIF1 alpha Antibody

Product Images

Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody

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  IHC analysis of HIF1Alpha tested on mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Specifications

Product Data

• Clone Name: ESEE122
• Applications: IHC, WB
• Recommended Dilution: IHC: 1:1000-1:8000, IF/ICC: 8-21ug/ml
• Reactivities: Human, Mouse, Rat, Cow
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment corresponding to amino acids 329-530
• Formulation: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypoxia inducible factor 1 alpha subunit
• Database Link:
  NP_001521
  Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human
  UniProt ID Q16665
• Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia . The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1 is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1 is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1 degradation and lead to its stabilization. In addition, HIF-1 can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway .
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• Note: Specific for HIF1Alpha

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma