Sod2 Rabbit Polyclonal Antibody

CAT#: TA326596

Rabbit Polyclonal Anti-SOD (Mn) Antibody

Product Images

Western blot analysis of Mn SOD in rat tissue lysates, using a 1:1000 dilution of the antibody

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  Mn SOD visualized using the antibody

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: 0.5ug/ml was sufficient for detection of Mn SOD in 20ug of rat brain tissue extract
• Reactivities: Human, Rat, Mouse, Bovine, Canine, Chicken, Dosophila, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat Mn SOD
• Formulation: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Affinity (antigen) Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: superoxide dismutase 2, mitochondrial
• Database Link:
  NP_058747
  Entrez Gene 6648 HumanEntrez Gene 20656 MouseEntrez Gene 574097 MonkeyEntrez Gene 24787 Rat
  UniProt ID P07895
• Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body . It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase . In general, SODs play a major role in antioxidant defense mechanisms . There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge . The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space . SOD3 can also be distinguished by its heparin-binding capacity .
• Synonyms: IPO-B; Mn-SOD; MNSOD; MVCD6
• Note: Detects a ~25kDa protein corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS PAGE immunoblots.