Osteopontin (SPP1) Rabbit Polyclonal Antibody

CAT#: TA326641

Rabbit Polyclonal SPP1 Antibody

Product Images

Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 ug/mL.

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  Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.
• Formulation: SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: SPP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 36 kDa; Observed: 37 kDa
• Gene Name: secreted phosphoprotein 1
• Database Link:
  NP_001035149
  Entrez Gene 20750 MouseEntrez Gene 25353 RatEntrez Gene 6696 Human
  UniProt ID P10451
• Background: SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).
• Synonyms: BNSP; BSPI; ETA-1; OPN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway