Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA326660

Rabbit Polyclonal Vinculin Antibody

Product Images

Western blot analysis of Vinculin in PC-3 cell lysate with Vinculin antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of Vinculin in rat small intestine tissue with Vinculin antibody at 5 ug/mL.

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  Immunofluorescence of Vinculin in rat small intestine tissue with Vinculin antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Vinculin antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Vinculin.
• Formulation: Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Vinculin Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 117 kDa; Observed: 120 kDa
• Gene Name: vinculin
• Database Link:
  NP_054706
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
• Synonyms: CMD1W; CMH15; HEL114; MV; MVCL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton